cartVersion cartVersion cartVersion cartVersion 0 0 0 0 0 0 0 0 0 0 0 cartVersion cartVersion cartVersion 0 cartVersion 0 geneMapper GeneMapper genePred GeneMapper Predictions from UCB 0 37 128 0 0 191 127 127 0 0 0

Description

\

\ This track shows gene predictions of \ GeneMapper, a program for transferring gene\ annotations (including UTRs) from a reference genome to newly\ sequenced genomes. These annotations have been obtained by\ transferring D. melanogaster RefSeq genes to the \ D. pseudoobscura genome.\

\ \

Methods

\

\ Whole genome homology maps of 9 fruitfly genomes were obtained using\ Colin Dewey's\ Mercator program. The homology map was used as a\ guide to transfer D. melanogaster RefSeq genes to the other \ genomes.\

\ \

References

\

\ Chatterji S, Pachter L.\ \ Reference based annotation with GeneMapper.\ Genome Biol. 2006;7(4):R29.\ PMID: 16600017; PMC: PMC1557983\

\ \

Credits

\

\ Thanks to Sourav Chatterji and Lior Pachter at U. C. Berkeley \ for providing these annotations.\

\ \ genes 1 color 128,0,0\ group genes\ longLabel GeneMapper Predictions from UCB\ priority 37\ shortLabel GeneMapper\ track geneMapper\ type genePred\ visibility hide\ intronEst Spliced ESTs psl est D. pseudoobscura ESTs That Have Been Spliced 1 56 0 0 0 127 127 127 1 0 0

Description

\

\ This track shows alignments between D. pseudoobscura expressed sequence tags\ (ESTs) in GenBank and the genome that show signs of splicing when\ aligned against the genome. ESTs are single-read sequences, typically about \ 500 bases in length, that usually represent fragments of transcribed genes.\

\

\ To be considered spliced, an EST must show \ evidence of at least one canonical intron, i.e. one that is at least\ 32 bases in length and has GT/AG ends. By requiring splicing, the level \ of contamination in the EST databases is drastically reduced\ at the expense of eliminating many genuine 3' ESTs.\ For a display of all ESTs (including unspliced), see the \ D. pseudoobscura EST track.

\ \

Display Conventions and Configuration

\

\ This track follows the display conventions for \ PSL alignment tracks. In dense display mode, darker shading\ indicates a larger number of aligned ESTs.

\

\ The strand information (+/-) indicates the\ direction of the match between the EST and the matching\ genomic sequence. It bears no relationship to the direction\ of transcription of the RNA with which it might be associated.

\

\ The description page for this track has a filter that can be used to change \ the display mode, alter the color, and include/exclude a subset of items \ within the track. This may be helpful when many items are shown in the track \ display, especially when only some are relevant to the current task.

\

\ To use the filter:\

    \
  1. Type a term in one or more of the text boxes to filter the EST\ display. For example, to apply the filter to all ESTs expressed in a specific\ organ, type the name of the organ in the tissue box. To view the list of \ valid terms for each text box, consult the table in the Table Browser that \ corresponds to the factor on which you wish to filter. For example, the \ "tissue" table contains all the types of tissues that can be \ entered into the tissue text box. Wildcards may also be used in the\ filter.\
  2. If filtering on more than one value, choose the desired combination\ logic. If "and" is selected, only ESTs that match all filter \ criteria will be highlighted. If "or" is selected, ESTs that \ match any one of the filter criteria will be highlighted.\
  3. Choose the color or display characteristic that should be used to \ highlight or include/exclude the filtered items. If "exclude" is \ chosen, the browser will not display ESTs that match the filter criteria. \ If "include" is selected, the browser will display only those \ ESTs that match the filter criteria.\

\

\ This track may also be configured to display base labeling, a feature that\ allows the user to display all bases in the aligning sequence or only those \ that differ from the genomic sequence. For more information about this option,\ click \ here.\

\ \

Methods

\

\ To make an EST, RNA is isolated from cells and reverse\ transcribed into cDNA. Typically, the cDNA is cloned\ into a plasmid vector and a read is taken from the 5'\ and/or 3' primer. For most — but not all — ESTs, the\ reverse transcription is primed by an oligo-dT, which\ hybridizes with the poly-A tail of mature mRNA. The\ reverse transcriptase may or may not make it to the 5'\ end of the mRNA, which may or may not be degraded.

\

\ In general, the 3' ESTs mark the end of transcription\ reasonably well, but the 5' ESTs may end at any point\ within the transcript. Some of the newer cap-selected\ libraries cover transcription start reasonably well. Before the \ cap-selection techniques\ emerged, some projects used random rather than poly-A\ priming in an attempt to retrieve sequence distant from the\ 3' end. These projects were successful at this, but as\ a side effect also deposited sequences from unprocessed\ mRNA and perhaps even genomic sequences into the EST databases.\ Even outside of the random-primed projects, there is a\ degree of non-mRNA contamination. Because of this, a\ single unspliced EST should be viewed with considerable\ skepticism.

\

\ To generate this track, D. pseudoobscura ESTs from GenBank were aligned \ against the genome using blat. Note that the maximum intron length\ allowed by blat is 750,000 bases, which may eliminate some ESTs with very \ long introns that might otherwise align. When a single \ EST aligned in multiple places, the alignment having the \ highest base identity was identified. Only alignments having\ a base identity level within 0.5% of the best and at least 96% base identity \ with the genomic sequence are displayed in this track.

\ \

Credits

\

\ This track was produced at UCSC from EST sequence data\ submitted to the international public sequence databases by \ scientists worldwide.

\ \

References

\

\ Benson DA, Karsch-Mizrachi I, Lipman DJ, Ostell J, Wheeler DL. \ GenBank: update.\ Nucleic Acids Res. 2004 Jan 1;32(Database issue):D23-6.\ PMID: 14681350; PMC: PMC308779\

\ \

\ Kent WJ.\ BLAT - the BLAST-like alignment tool.\ Genome Res. 2002 Apr;12(4):656-64.\ PMID: 11932250; PMC: PMC187518\

\ rna 1 baseColorUseSequence genbank\ group rna\ indelDoubleInsert on\ indelQueryInsert on\ intronGap 30\ longLabel D. pseudoobscura ESTs That Have Been Spliced\ priority 56\ shortLabel Spliced ESTs\ showDiffBasesAllScales .\ spectrum on\ track intronEst\ type psl est\ visibility dense\ augustusGene AUGUSTUS genePred AUGUSTUS ab initio gene predictions v3.1 0 100 12 105 0 133 180 127 0 0 0

Description

\ \

\ This track shows ab initio predictions from the program\ AUGUSTUS (version 3.1).\ The predictions are based on the genome sequence alone.\

\ \

\ For more information on the different gene tracks, see our Genes FAQ.

\ \

Methods

\ \

\ Statistical signal models were built for splice sites, branch-point\ patterns, translation start sites, and the poly-A signal.\ Furthermore, models were built for the sequence content of\ protein-coding and non-coding regions as well as for the length distributions\ of different exon and intron types. Detailed descriptions of most of these different models\ can be found in Mario Stanke's\ dissertation.\ This track shows the most likely gene structure according to a\ Semi-Markov Conditional Random Field model.\ Alternative splicing transcripts were obtained with\ a sampling algorithm (--alternatives-from-sampling=true --sample=100 --minexonintronprob=0.2\ --minmeanexonintronprob=0.5 --maxtracks=3 --temperature=2).\

\ \

\ The different models used by Augustus were trained on a number of different species-specific\ gene sets, which included 1000-2000 training gene structures. The --species option allows\ one to choose the species used for training the models. Different training species were used\ for the --species option when generating these predictions for different groups of\ assemblies.\ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \ \
Assembly GroupTraining Species
Fishzebrafish\ \
Birdschicken\ \
Human and all other vertebrateshuman\ \
Nematodescaenorhabditis
Drosophilafly
A. melliferahoneybee1
A. gambiaeculex
S. cerevisiaesaccharomyces
\

\ This table describes which training species was used for a particular group of assemblies.\ When available, the closest related training species was used.\

\ \

Credits

\ \ Thanks to the\ Stanke lab\ for providing the AUGUSTUS program. The training for the chicken version was\ done by Stefanie König and the training for the\ human and zebrafish versions was done by Mario Stanke.\ \

References

\ \

\ Stanke M, Diekhans M, Baertsch R, Haussler D.\ \ Using native and syntenically mapped cDNA alignments to improve de novo gene finding.\ Bioinformatics. 2008 Mar 1;24(5):637-44.\ PMID: 18218656\

\ \

\ Stanke M, Waack S.\ \ Gene prediction with a hidden Markov model and a new intron submodel.\ Bioinformatics. 2003 Oct;19 Suppl 2:ii215-25.\ PMID: 14534192\

\ genes 1 baseColorDefault genomicCodons\ baseColorUseCds given\ color 12,105,0\ group genes\ longLabel AUGUSTUS ab initio gene predictions v3.1\ shortLabel AUGUSTUS\ track augustusGene\ type genePred\ visibility hide\ blastDm1FB D. mel. Proteins psl protein D. melanogaster Proteins 3 100 0 0 0 127 127 127 0 0 0 http://flybase.bio.indiana.edu/.bin/fbidq.html?

Description

\

\ This track contains tBLASTn alignments of the peptides\ from the predicted and known genes identified in the D. melanogaster\ FlyBase as of 24 July 2004 to the D. pseudoobscura sequence.\ \

Methods

\

\ First, predicted proteins from the D. melanogaster FlyBase track were\ aligned with the D. melanogaster genome using the blat program to \ discover exon boundaries. \ Next, the amino acid sequences that make up each exon were aligned with the \ D. pseudoobscura sequence using the tBLASTn program.\ Finally, the putative D. pseudoobscura exons were chained together using an \ organism-specific maximum gap size but no gap penalty. The single best exon \ chains extending over more than 60% of the query protein were included. Exon \ chains that extended over 60% of the query and matched at least 60% of the \ protein's amino acids were also included.\ \

Credits

\

\ tBLASTn is part of the NCBI Blast tool set. For more information on Blast, see\ Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ.\ Basic local alignment search tool.\ J Mol Biol. 1990 Oct 5;215(3):403-10.\ PMID: 2231712\

\ \

\ Blat was written by Jim Kent. The remaining utilities \ required to produce this track were written by Jim Kent or Brian Raney.\ genes 1 blastRef dm1.blastFBRef00\ colorChromDefault off\ group genes\ longLabel D. melanogaster Proteins\ pred dm1.blastFBPep00\ shortLabel D. mel. Proteins\ track blastDm1FB\ type psl protein\ url http://flybase.bio.indiana.edu/.bin/fbidq.html?\ visibility pack\ est D. pseudo. ESTs psl est D. pseudoobscura ESTs Including Unspliced 0 100 0 0 0 127 127 127 1 0 0

Description

\

\ This track shows alignments between D. pseudoobscura expressed sequence tags\ (ESTs) in GenBank and the genome. ESTs are single-read sequences, \ typically about 500 bases in length, that usually represent fragments of \ transcribed genes.

\ \

Display Conventions and Configuration

\

\ This track follows the display conventions for \ PSL alignment tracks. In dense display mode, the items that\ are more darkly shaded indicate matches of better quality.

\

\ The strand information (+/-) indicates the\ direction of the match between the EST and the matching\ genomic sequence. It bears no relationship to the direction\ of transcription of the RNA with which it might be associated.

\

\ The description page for this track has a filter that can be used to change \ the display mode, alter the color, and include/exclude a subset of items \ within the track. This may be helpful when many items are shown in the track \ display, especially when only some are relevant to the current task.

\

\ To use the filter:\

    \
  1. Type a term in one or more of the text boxes to filter the EST\ display. For example, to apply the filter to all ESTs expressed in a specific\ organ, type the name of the organ in the tissue box. To view the list of \ valid terms for each text box, consult the table in the Table Browser that \ corresponds to the factor on which you wish to filter. For example, the \ "tissue" table contains all the types of tissues that can be \ entered into the tissue text box. Wildcards may also be used in the\ filter.\
  2. If filtering on more than one value, choose the desired combination\ logic. If "and" is selected, only ESTs that match all filter \ criteria will be highlighted. If "or" is selected, ESTs that \ match any one of the filter criteria will be highlighted.\
  3. Choose the color or display characteristic that should be used to \ highlight or include/exclude the filtered items. If "exclude" is \ chosen, the browser will not display ESTs that match the filter criteria. \ If "include" is selected, the browser will display only those \ ESTs that match the filter criteria.\

\

\ This track may also be configured to display base labeling, a feature that\ allows the user to display all bases in the aligning sequence or only those \ that differ from the genomic sequence. For more information about this option,\ click \ here.\

\ \

Methods

\

\ To make an EST, RNA is isolated from cells and reverse\ transcribed into cDNA. Typically, the cDNA is cloned\ into a plasmid vector and a read is taken from the 5'\ and/or 3' primer. For most — but not all — ESTs, the\ reverse transcription is primed by an oligo-dT, which\ hybridizes with the poly-A tail of mature mRNA. The\ reverse transcriptase may or may not make it to the 5'\ end of the mRNA, which may or may not be degraded.

\

\ In general, the 3' ESTs mark the end of transcription\ reasonably well, but the 5' ESTs may end at any point\ within the transcript. Some of the newer cap-selected\ libraries cover transcription start reasonably well. Before the \ cap-selection techniques\ emerged, some projects used random rather than poly-A\ priming in an attempt to retrieve sequence distant from the\ 3' end. These projects were successful at this, but as\ a side effect also deposited sequences from unprocessed\ mRNA and perhaps even genomic sequences into the EST databases.\ Even outside of the random-primed projects, there is a\ degree of non-mRNA contamination. Because of this, a\ single unspliced EST should be viewed with considerable\ skepticism.

\

\ To generate this track, D. pseudoobscura ESTs from GenBank were aligned \ against the genome using blat. Note that the maximum intron length\ allowed by blat is 750,000 bases, which may eliminate some ESTs with very \ long introns that might otherwise align. When a single \ EST aligned in multiple places, the alignment having the \ highest base identity was identified. Only alignments having\ a base identity level within 0.5% of the best and at least 96% base identity \ with the genomic sequence are displayed in this track.

\ \

Credits

\

\ This track was produced at UCSC from EST sequence data\ submitted to the international public sequence databases by \ scientists worldwide.

\ \

References

\

\ Benson DA, Karsch-Mizrachi I, Lipman DJ, Ostell J, Wheeler DL.\ GenBank: update.\ Nucleic Acids Res. 2004 Jan 1;32(Database issue):D23-6.\ PMID: 14681350; PMC: PMC308779\

\ \

\ Kent WJ.\ BLAT - the BLAST-like alignment tool.\ Genome Res. 2002 Apr;12(4):656-64.\ PMID: 11932250; PMC: PMC187518\

\ rna 1 baseColorUseSequence genbank\ group rna\ indelDoubleInsert on\ indelQueryInsert on\ intronGap 30\ longLabel D. pseudoobscura ESTs Including Unspliced\ maxItems 300\ shortLabel D. pseudo. ESTs\ spectrum on\ table all_est\ track est\ type psl est\ visibility hide\ mrna D. pseudo. mRNAs psl . D. pseudoobscura mRNAs from GenBank 1 100 0 0 0 127 127 127 0 0 0

Description

\

\ The mRNA track shows alignments between D. pseudoobscura mRNAs\ in GenBank and the genome.

\ \

Display Conventions and Configuration

\

\ This track follows the display conventions for \ PSL alignment tracks. In dense display mode, the items that\ are more darkly shaded indicate matches of better quality.

\

\ The description page for this track has a filter that can be used to change \ the display mode, alter the color, and include/exclude a subset of items \ within the track. This may be helpful when many items are shown in the track \ display, especially when only some are relevant to the current task.

\

\ To use the filter:\

    \
  1. Type a term in one or more of the text boxes to filter the mRNA \ display. For example, to apply the filter to all mRNAs expressed in a specific\ organ, type the name of the organ in the tissue box. To view the list of \ valid terms for each text box, consult the table in the Table Browser that \ corresponds to the factor on which you wish to filter. For example, the \ "tissue" table contains all the types of tissues that can be \ entered into the tissue text box. Wildcards may also be used in the\ filter.\
  2. If filtering on more than one value, choose the desired combination\ logic. If "and" is selected, only mRNAs that match all filter \ criteria will be highlighted. If "or" is selected, mRNAs that \ match any one of the filter criteria will be highlighted.\
  3. Choose the color or display characteristic that should be used to \ highlight or include/exclude the filtered items. If "exclude" is \ chosen, the browser will not display mRNAs that match the filter criteria. \ If "include" is selected, the browser will display only those \ mRNAs that match the filter criteria.\

\

\ This track may also be configured to display codon coloring, a feature that\ allows the user to quickly compare mRNAs against the genomic sequence. For more \ information about this option, click \ here.\

\ \

Methods

\

\ GenBank D. pseudoobscura mRNAs were aligned against the genome using the \ blat program. When a single mRNA aligned in multiple places, \ the alignment having the highest base identity was found. \ Only alignments having a base identity level within 0.5% of\ the best and at least 96% base identity with the genomic sequence were kept.\

\ \

Credits

\

\ The mRNA track was produced at UCSC from mRNA sequence data\ submitted to the international public sequence databases by \ scientists worldwide.

\ \

References

\

\ Benson DA, Karsch-Mizrachi I, Lipman DJ, Ostell J, Wheeler DL.\ GenBank: update.\ Nucleic Acids Res. 2004 Jan 1;32(Database issue):D23-6.\ PMID: 14681350; PMC: PMC308779\

\ \

\ Kent WJ.\ BLAT - the BLAST-like alignment tool.\ Genome Res. 2002 Apr;12(4):656-64.\ PMID: 11932250; PMC: PMC187518\

\ \ rna 1 baseColorDefault diffCodons\ baseColorUseCds genbank\ baseColorUseSequence genbank\ group rna\ indelDoubleInsert on\ indelPolyA on\ indelQueryInsert on\ longLabel D. pseudoobscura mRNAs from GenBank\ shortLabel D. pseudo. mRNAs\ showDiffBasesAllScales .\ table all_mrna\ track mrna\ type psl .\ visibility dense\ gap Gap bed 3 + Gap Locations 1 100 0 0 0 127 127 127 0 0 0

Description

\ This track depicts gaps in the assembly. These gaps - with the\ exception of intractable heterochromatic gaps - will be closed during the\ finishing process. \

\ Gaps are represented as black boxes in this track.\ If the relative order and orientation of the contigs on either side\ of the gap is known, it is a bridged gap and a white line is drawn \ through the black box representing the gap. \

\ Gap locations were extracted from the sequence by looking for \ stretches of at least 10 consecutive N bases. \

\

Gaps were considered to be one of these two types, based on length:\

\ map 1 group map\ longLabel Gap Locations\ shortLabel Gap\ track gap\ type bed 3 +\ visibility dense\ gcPercent GC Percent bed 4 + Percentage GC in 20,000-Base Windows 0 100 0 0 0 127 127 127 1 0 0

Description

\

\ The GC percent track shows the percentage of G (guanine) and C (cytosine) bases\ in a 20,000 base window. Windows with high GC content are drawn more darkly \ than windows with low GC content. High GC content is typically associated with \ gene-rich areas.\

\

Credits

\

\ This track was generated at UCSC.\ map 1 group map\ longLabel Percentage GC in 20,000-Base Windows\ shortLabel GC Percent\ spectrum on\ track gcPercent\ type bed 4 +\ visibility hide\ genscan Genscan Genes genePred genscanPep Genscan Gene Predictions 1 100 170 100 0 212 177 127 0 0 0

Description

\ \

\ This track shows predictions from the\ Genscan program\ written by Chris Burge.\ The predictions are based on transcriptional, translational and donor/acceptor\ splicing signals as well as the length and compositional distributions of exons,\ introns and intergenic regions.\

\ \

\ For more information on the different gene tracks, see our Genes FAQ.

\ \

Display Conventions and Configuration

\ \

\ This track follows the display conventions for\ gene prediction\ tracks.\

\ \

\ The track description page offers the following filter and configuration\ options:\

\

\ \

Methods

\ \

\ For a description of the Genscan program and the model that underlies it,\ refer to Burge and Karlin (1997) in the References section below.\ The splice site models used are described in more detail in Burge (1998)\ below.\

\ \

Credits

\ \ Thanks to Chris Burge for providing the Genscan program.\ \

References

\ \

\ Burge C.\ Modeling Dependencies in Pre-mRNA Splicing Signals.\ In: Salzberg S, Searls D, Kasif S, editors.\ Computational Methods in Molecular Biology.\ Amsterdam: Elsevier Science; 1998. p. 127-163.\

\ \

\ Burge C, Karlin S.\ \ Prediction of complete gene structures in human genomic DNA.\ J. Mol. Biol. 1997 Apr 25;268(1):78-94.\ PMID: 9149143\

\ genes 1 color 170,100,0\ group genes\ longLabel Genscan Gene Predictions\ shortLabel Genscan Genes\ track genscan\ type genePred genscanPep\ visibility dense\ microsat Microsatellite bed 4 Microsatellites - Di-nucleotide and Tri-nucleotide Repeats 0 100 0 0 0 127 127 127 0 0 0

Description

\

\ This track displays regions that are likely to be useful as microsatellite\ markers. These are sequences of at least 15 perfect di-nucleotide and \ tri-nucleotide repeats and tend to be highly polymorphic in the\ population.\

\ \

Methods

\

\ The data shown in this track are a subset of the Simple Repeats track, \ selecting only those \ repeats of period 2 and 3, with 100% identity and no indels and with\ at least 15 copies of the repeat. The Simple Repeats track is\ created using the \ Tandem Repeats Finder. For more information about this \ program, see Benson (1999).

\ \

Credits

\

\ Tandem Repeats Finder was written by \ Gary Benson.

\ \

References

\ \

\ Benson G.\ \ Tandem repeats finder: a program to analyze DNA sequences.\ Nucleic Acids Res. 1999 Jan 15;27(2):573-80.\ PMID: 9862982; PMC: PMC148217\

\ varRep 1 group varRep\ longLabel Microsatellites - Di-nucleotide and Tri-nucleotide Repeats\ shortLabel Microsatellite\ track microsat\ type bed 4\ visibility hide\ xenoRefGene Other RefSeq genePred xenoRefPep xenoRefMrna Non-D. pseudoobscura RefSeq Genes 1 100 12 12 120 133 133 187 0 0 0

Description

\

\ This track shows known protein-coding and non-protein-coding genes \ for organisms other than D. pseudoobscura, taken from the NCBI RNA reference\ sequences collection (RefSeq). The data underlying this track are \ updated weekly.

\ \

Display Conventions and Configuration

\

\ This track follows the display conventions for \ gene prediction \ tracks.\ The color shading indicates the level of review the RefSeq record has \ undergone: predicted (light), provisional (medium), reviewed (dark).

\

\ The item labels and display colors of features within this track can be\ configured through the controls at the top of the track description page. \

\ \

Methods

\

\ The RNAs were aligned against the D. pseudoobscura genome using blat; those\ with an alignment of less than 15% were discarded. When a single RNA aligned \ in multiple places, the alignment having the highest base identity was \ identified. Only alignments having a base identity level within 0.5% of \ the best and at least 25% base identity with the genomic sequence were kept.\

\ \

Credits

\

\ This track was produced at UCSC from RNA sequence data\ generated by scientists worldwide and curated by the \ NCBI RefSeq project.

\ \

References

\

\ Kent WJ.\ BLAT - the BLAST-like alignment tool.\ Genome Res. 2002 Apr;12(4):656-64.\ PMID: 11932250; PMC: PMC187518\

\ genes 1 color 12,12,120\ group genes\ longLabel Non-D. pseudoobscura RefSeq Genes\ shortLabel Other RefSeq\ track xenoRefGene\ type genePred xenoRefPep xenoRefMrna\ visibility dense\ simpleRepeat Simple Repeats bed 4 + Simple Tandem Repeats by TRF 0 100 0 0 0 127 127 127 0 0 0

Description

\

\ This track displays simple tandem repeats (possibly imperfect repeats) located\ by Tandem Repeats\ Finder (TRF) which is specialized for this purpose. These repeats can\ occur within coding regions of genes and may be quite\ polymorphic. Repeat expansions are sometimes associated with specific\ diseases.

\ \

Methods

\

\ For more information about the TRF program, see Benson (1999).\

\ \

Credits

\

\ TRF was written by \ Gary Benson.

\ \

References

\ \

\ Benson G.\ \ Tandem repeats finder: a program to analyze DNA sequences.\ Nucleic Acids Res. 1999 Jan 15;27(2):573-80.\ PMID: 9862982; PMC: PMC148217\

\ varRep 1 group varRep\ longLabel Simple Tandem Repeats by TRF\ shortLabel Simple Repeats\ track simpleRepeat\ type bed 4 +\ visibility hide\ twinscan Twinscan genePred twinscanPep Twinscan Gene Predictions Using Mouse/Human Homology 0 100 0 100 100 127 177 177 0 0 0

Description

\

\ The Twinscan program predicts genes in a manner similar to Genscan, except \ that Twinscan takes advantage of genome comparisons to improve gene prediction\ accuracy. More information and a web server can be found at\ http://mblab.wustl.edu/.

\ \

Display Conventions and Configuration

\

\ This track follows the display conventions for \ gene prediction \ tracks.

\

\ The track description page offers the following filter and configuration\ options:\

\ \

Methods

\

\ The Twinscan algorithm is described in Korf, I. et al. (2001) in the\ References section below.

\ \

Credits

\

\ Thanks to Michael Brent's Computational Genomics Group at Washington \ University St. Louis for providing these data.

\ \

References

\

\ Korf I, Flicek P, Duan D, Brent MR.\ Integrating genomic homology into gene structure prediction.\ Bioinformatics. 2001;17 Suppl 1:S140-8.\ PMID: 11473003\

\ genes 1 color 0,100,100\ group genes\ longLabel Twinscan Gene Predictions Using Mouse/Human Homology\ shortLabel Twinscan\ track twinscan\ type genePred twinscanPep\ visibility hide\ chainDm1 D. mel. Chain chain dm1 D. melanogaster (Jan. 2003 (BDGP R3/dm1)) Chained Alignments 0 133 100 50 0 255 240 200 1 0 0

Description

\

\ This track shows alignments of D. melanogaster (dm1, Jan. 2003 (BDGP R3/dm1)) to the\ D. pseudoobscura genome using a gap scoring system that allows longer gaps \ than traditional affine gap scoring systems. It can also tolerate gaps in both\ D. melanogaster and D. pseudoobscura simultaneously. These \ "double-sided" gaps can be caused by local inversions and \ overlapping deletions in both species. \

\ The chain track displays boxes joined together by either single or\ double lines. The boxes represent aligning regions.\ Single lines indicate gaps that are largely due to a deletion in the\ D. melanogaster assembly or an insertion in the D. pseudoobscura \ assembly. Double lines represent more complex gaps that involve substantial\ sequence in both species. This may result from inversions, overlapping\ deletions, an abundance of local mutation, or an unsequenced gap in one\ species. In cases where multiple chains align over a particular region of\ the D. pseudoobscura genome, the chains with single-lined gaps are often \ due to processed pseudogenes, while chains with double-lined gaps are more \ often due to paralogs and unprocessed pseudogenes.

\

\ In the "pack" and "full" display\ modes, the individual feature names indicate the chromosome, strand, and\ location (in thousands) of the match for each matching alignment.

\ \ \

Display Conventions and Configuration

\

By default, the chains to chromosome-based assemblies are colored\ based on which chromosome they map to in the aligning organism. To turn\ off the coloring, check the "off" button next to: Color\ track based on chromosome.

\

\ To display only the chains of one chromosome in the aligning\ organism, enter the name of that chromosome (e.g. chr4) in box next to: \ Filter by chromosome.

\ \

Methods

\

\ Transposons that have been inserted since the \ D. melanogaster/D. pseudoobscura\ split were removed from the assemblies. The abbreviated genomes were\ aligned with blastz, and the transposons were then added back in.\ The resulting alignments were converted into axt format using the lavToAxt\ program. The axt alignments were fed into axtChain, which organizes all\ alignments between a single D. melanogaster chromosome and a single\ D. pseudoobscura chromosome into a group and creates a kd-tree out\ of the gapless subsections (blocks) of the alignments. A dynamic program\ was then run over the kd-trees to find the maximally scoring chains of these\ blocks. Chains scoring below a threshold were discarded; the remaining\ chains are displayed in this track.

\ \

Credits

\

\ Blastz was developed at Pennsylvania State University by \ Minmei Hou, Scott Schwartz, Zheng Zhang, and Webb Miller with advice from\ Ross Hardison.

\

\ Lineage-specific repeats were identified by Arian Smit and his \ RepeatMasker\ program.

\

\ The axtChain program was developed at the University of California at \ Santa Cruz by Jim Kent with advice from Webb Miller and David Haussler.

\

\ The browser display and database storage of the chains were generated\ by Robert Baertsch and Jim Kent.

\ \

References

\

\ Chiaromonte F, Yap VB, Miller W.\ Scoring pairwise genomic sequence alignments.\ Pac Symp Biocomput. 2002:115-26.\ PMID: 11928468\

\ \

\ Kent WJ, Baertsch R, Hinrichs A, Miller W, Haussler D.\ Evolution's cauldron: Duplication, deletion, and rearrangement\ in the mouse and human genomes.\ Proc Natl Acad Sci U S A. 2003 Sep 30;100(20):11484-9.\ PMID: 14500911; PMC: PMC208784\

\ \

\ Schwartz S, Kent WJ, Smit A, Zhang Z, Baertsch R, Hardison R,\ Haussler D, Miller W.\ Human-Mouse Alignments with BLASTZ.\ Genome Res. 2003 Jan;13(1):103-7.\ PMID: 12529312; PMC: PMC430961\

\ compGeno 1 altColor 255,240,200\ color 100,50,0\ group compGeno\ longLabel D. melanogaster (Jan. 2003 (BDGP R3/dm1)) Chained Alignments\ otherDb dm1\ priority 133\ shortLabel D. mel. Chain\ spectrum on\ track chainDm1\ type chain dm1\ visibility hide\ netDm1 D. mel. Net netAlign dm1 chainDm1 D. melanogaster (Jan. 2003 (BDGP R3/dm1)) Alignment Net 1 134 0 0 0 127 127 127 1 0 0

Description

\

\ This track shows the best D. melanogaster/D. pseudoobscura \ chain for every part of the D. pseudoobscura genome. It is useful for\ finding orthologous regions and for studying genome rearrangement.\ The D. melanogaster sequence used in this annotation is \ from the Jan. 2003 (BDGP R3/dm1) (dm1) assembly.

\ \

Display Conventions and Configuration

\

\ In full display mode, the top-level (level 1)\ chains are the largest, highest-scoring chains that\ span this region. In many cases gaps exist in the\ top-level chain. When possible, these are filled in by\ other chains that are displayed at level 2. The gaps in \ level 2 chains may be filled by level 3 chains and so\ forth.

\

\ In the graphical display, the boxes represent ungapped \ alignments; the lines represent gaps. Click\ on a box to view detailed information about the chain\ as a whole; click on a line to display information\ about the gap. The detailed information is useful in determining\ the cause of the gap or, for lower level chains, the genomic\ rearrangement.

\

\ Individual items in the display are categorized as one of four types\ (other than gap):

\

\ \

Methods

\

\ Chains were derived from blastz alignments, using the methods\ described on the chain tracks description pages, and sorted with the \ highest-scoring chains in the genome ranked first. The program\ chainNet was then used to place the chains one at a time, trimming them as \ necessary to fit into sections not already covered by a higher-scoring chain. \ During this process, a natural hierarchy emerged in which a chain that filled \ a gap in a higher-scoring chain was placed underneath that chain. The program \ netSyntenic was used to fill in information about the relationship between \ higher- and lower-level chains, such as whether a lower-level\ chain was syntenic or inverted relative to the higher-level chain. \ The program netClass was then used to fill in how much of the gaps and chains \ contained Ns (sequencing gaps) in one or both species and how much\ was filled with transposons inserted before and after the two organisms \ diverged.

\ \

Credits

\

\ The chainNet, netSyntenic, and netClass programs were\ developed at the University of California\ Santa Cruz by Jim Kent.

\

\ Blastz was developed at Pennsylvania State University by\ Minmei Hou, Scott Schwartz, Zheng Zhang, and Webb Miller with advice from\ Ross Hardison.

\

\ Lineage-specific repeats were identified by Arian Smit and his program \ RepeatMasker.

\

\ The browser display and database storage of the nets were made\ by Robert Baertsch and Jim Kent.

\ \

References

\

\ Kent WJ, Baertsch R, Hinrichs A, Miller W, Haussler D.\ Evolution's cauldron: Duplication, deletion, and rearrangement\ in the mouse and human genomes.\ Proc Natl Acad Sci U S A. 2003 Sep 30;100(20):11484-9.\ PMID: 14500911; PMC: PMC208784\

\ \

\ Schwartz S, Kent WJ, Smit A, Zhang Z, Baertsch R, Hardison R,\ Haussler D, Miller W.\ Human-Mouse Alignments with BLASTZ.\ Genome Res. 2003 Jan;13(1):103-7.\ PMID: 12529312; PMC: PMC430961\

\ compGeno 0 group compGeno\ longLabel D. melanogaster (Jan. 2003 (BDGP R3/dm1)) Alignment Net\ otherDb dm1\ priority 134\ shortLabel D. mel. Net\ spectrum on\ track netDm1\ type netAlign dm1 chainDm1\ visibility dense\ rmsk RepeatMasker rmsk Repeating Elements by RepeatMasker 1 149.1 0 0 0 127 127 127 1 0 0

Description

\

\ This track was created by using Arian Smit's RepeatMasker program, which screens DNA sequences \ for interspersed repeats and low complexity DNA sequences. The program\ outputs a detailed annotation of the repeats that are present in the\ query sequence (represented by this track), as well as a modified version\ of the query sequence in which all the annotated repeats have been masked\ (generally available on the\ Downloads page). RepeatMasker uses \ the Repbase Update library of repeats from the \ Genetic \ Information Research Institute (GIRI). \ Repbase Update is described in Jurka, J. (2000) in the References section below.

\ \

Display Conventions and Configuration

\

\ In full display mode, this track displays up to ten different classes of repeats:\

\

\ The level of color shading in the graphical display reflects the amount of \ base mismatch, base deletion, and base insertion associated with a repeat \ element. The higher the combined number of these, the lighter the shading.

\ \

Methods

\

\ UCSC has used the most current versions of the RepeatMasker software \ and repeat libraries available to generate these data. Note that these \ versions may be newer than those that are publicly available on the Internet. \

\

\ Data are generated using the RepeatMasker -s flag. Additional flags\ may be used for certain organisms. Repeats are soft-masked. Alignments may \ extend through repeats, but are not permitted to initiate in them. \ See the \ FAQ for \ more information.

\ \

Credits

\

\ Thanks to Arian Smit and GIRI\ for providing the tools and repeat libraries used to generate this track.

\ \

References

\

\ Jurka J.\ Repbase update: a database and an electronic journal of repetitive elements.\ Trends Genet. 2000 Sep;16(9):418-20.\ PMID: 10973072\

\ varRep 0 canPack off\ group varRep\ longLabel Repeating Elements by RepeatMasker\ priority 149.1\ shortLabel RepeatMasker\ spectrum on\ track rmsk\ type rmsk\ visibility dense\